Comparison of Pfizer/BioNTech BNT162b2 COVID-19 (Comirnaty) Vaccination Effects in Aged Adults with Special Consideration for the Effectiveness of the Three-Color iSpot in Assessing Immune Response

Background: The roll-out of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was certainly among the fastest in medical history. Vaccination campaigns around world began a year after outbreak 2019. When Austria started vaccinating population 2020, we took opportunity to collect data from first sets patients receiving vaccine our study region East Tyrol. Purpose: Many studies have been conducted examining immunogenicity new using classic serological test methods combination with an IFN-γ ELISpot. Undeniable disadvantages characterize status cellular immunity are that 1) being phase cytokine, loses signal strength long run and 2) does not provide information about involvement T helper (Th2) cells immune process. This implies it can affect false negative cell-mediated status. Method: Therefore, addition chemiluminescent immunoassay enzymatic ELISpot, this included fluorescent ELISpot assay precoated human SARS-CoV-2-specific IFN-γ/IL-2/IL-5 kits show more holistic overview on 1 (Th1) as senders IL-2 Th2 IL-5. Results Conclusion: Our confirms good Pfizer/BioNTech BNT162b2 COVID-19 (Comirnaty) strong Th1 vanishingly small participation. three color iSpot improve diagnostic results’ significance for individual, especially when infection has longer past diminishes.